Bladder Cancer Surgery

In this study, we aimed to examine the expression of these transcription factors in human bladder carcinoma.
Methods:
We first investigated expression of Slug,Snail,Twist and E-cadherin in five bladder Carcinoma cell lines by reverse transcription-polymerase chain reaction and western blot. Furthermore,we investigated Slug,Snail,and Twist and E-cadherin expression by immunohistochemistry with bladder carcinoma (tumor, n = 120; background,n =42).
Results:
Expression of Slug mRNA and protein was detected in all cell lines, Twist was clearly expressed in two out of five bladder carcinoma cell lines, Snail was not expressed, and E-cadherin was detected in 3 cell lines. 44.2% (53/120) of human bladder Carcinoma tissues and 38%(16/42) background tissue showed an expression of Twist; 62.5%(75/120… Source: MedWorm: Bladder Cancer

The objective
of the present study was to investigate the tumoricidal potential of combining the apoptosis-inducing protein TNF-related
apoptosis-inducing ligand (TRAIL) with a small molecule inhibitor of apoptosis proteins (IAP) antagonist to interfere with
intracellular regulators of apoptosis in human bladder tumor cells. Our results demonstrate that the IAP antagonist Compound
A exhibits high binding affinity to the XIAP BIR3 domain. When Compound A was used at nontoxic concentrations in combination
with TRAIL, there was a significant increase in the sensitivity of TRAIL-sensitive and TRAIL-resistant bladder tumor lines
to TRAIL-mediated apoptosis. In addition, modulation of TRAIL sensitivity in the TRAIL-resistant bladder tumor cell line T24
with Compound A was reciprocated by … Source: MedWorm: Bladder Cancer

(Source: BJU International)

Source: MedWorm: Bladder Cancer

Bladder carcinoma is the second most common malignancy of the urinary tract and nearly 90% of all primary tumors of the bladder are urothelial carcinomas (UCs). Superficial UCs can be “shaved off” using an electrocautery device attached to a cystoscope. Immunotherapy in the form of bacillus Calmette-Guerrin (BCG) instillation is also used to treat and prevent the recurrence of superficial UCs, but there are still patients, whose UCs recurred after treatment with BCG. Therefore, the development of new drugs that target UC cells is desirable… (Source: Health News from Medical News Today) Source: MedWorm: Bladder Cancer

Homodimerization of RON (MST1R), a receptor tyrosine kinase, usually occurs in cells stimulated by a ligand and leads to the downstream activation of signaling pathways. Here we report that bladder cancer cells, in response to physiological stress, use an alternative mechanism for signaling activation. Time-course studies indicated that RON migrated directly from the membrane to the nucleus of bladder cancer cells in response to serum starvation. Biochemical and genetic studies implied that this nuclear internalization was complexed with epidermal growth factor receptor (EGFR) and required the docking of importins. In vivo analysis confirmed that nuclear RON was present in 38.4% (28/73) of primary bladder tumors. Chromatin immunoprecipitation (ChIP) on microarray analysis further revealed …

Source: MedWorm: Bladder Cancer

BERKELEY, CA (UroToday.com) – Bladder carcinoma is the second most common malignancy of the urinary tract and nearly 90% of… (Source: UroToday)

Source: MedWorm: Bladder Cancer

Abstract  Carcinoma cells become more motile and invasive via downmodulation of E-cadherin. Cyclooxygenase-2 (COX-2) expression is associated
with tumor invasion and metastasis. The aim of this study is to investigate the relationship between the expression of COX-2
and E-cadherin in a bladder cancer cell line and human bladder transitional cell carcinoma (TCCs). Phorbol 12-myristate 13-acetate
(PMA) treatment for 5637 bladder cancer cells increased COX-2 expression, slightly induced Slug expression, and decreased
E-cadherin expression. Ectopic expression of COX-2 or prostaglandin E2 (PGE2) treatment for 5637 cells reduced E-cadherin expression. This finding was confirmed by the result that knockdown of COX-2
expression or indomethacin administration increased the expression… Source: MedWorm: Bladder Cancer

Authors: Lei Y, Yan S, Ming-De L, Na L, Rui-Fa H
Aurora-A is an oncogenic serine/threonine kinase, which plays important roles in tumorigenesis, development and chemoresistance of human cancers. The aim of the study was to detect the expression of Aurora-A gene in bladder cancer tissues and analyze its association with prognosis of bladder cancer patients. RT-PCR was performed to detect the expression of Aurora-A mRNA in 20 cases of bladder cancer and corresponding non-tumor tissue samples. Immunohistochemistry was performed to detect the localization of Aurora-A protein in 96 cases of bladder cancer tissue samples. Associations between Aurora-A protein expression and clinico-pathological factors or survival of bladder cancer patients were statistically analyzed. It was found that the … Source: MedWorm: Bladder Cancer

CONCLUSIONS: VEGFR-2 and VEGFR-3 are primarily localized to, and significantly upregulated on, tumor vasculature (blood and/or lymphatic) supporting the majority of solid cancers. The primary clinical mechanism of action of VEGF signaling inhibitors is likely to be through the targeting of tumor vessels rather than tumor cells. The upregulation of VEGFR-3 on tumor blood vessels indicates a potential additional antiangiogenic effect for dual VEGFR-2/VEGFR-3-targeted therapy. Clin Cancer Res; 16(14); 3548-61. (c)2010 AACR.
PMID: 20606037 [PubMed - as supplied by publisher] (Source: Clinical Cancer Research) Source: MedWorm: Bladder Cancer

Conclusion: This is the first study to demonstrate that PT causes autophagy in cancer cells and suggests that PT could serve as a new and promising agent for the treatment of sensitive and chemoresistant bladder cancer cells. (Source: Molecular Nutrition) Source: MedWorm: Bladder Cancer