Bladder Cancer Surgery

Many details of the molecular and cellular mechanisms involved in Mycobacterium bovis bacillus Calmette-Guérin (BCG) immunotherapy of bladder cancer have been discovered in the past decades. However, information on a potential role for macrophage cytotoxicity as an effector mechanism is limited. Macrophages play pivotal roles in the host innate immunity and serve as a first line of defense in mycobacterial infection. In addition to their function as professional antigen-presenting cells, the tumoricidal activity of macrophages has also been studied with considerable interest. Studies have shown that activated macrophages are potent in killing malignant cells of various tissue origins. This review summarizes the current understanding of the BCG-induced macrophage cytotoxicity toward bl…

Source: MedWorm: Bladder Cancer

The objective
of the present study was to investigate the tumoricidal potential of combining the apoptosis-inducing protein TNF-related
apoptosis-inducing ligand (TRAIL) with a small molecule inhibitor of apoptosis proteins (IAP) antagonist to interfere with
intracellular regulators of apoptosis in human bladder tumor cells. Our results demonstrate that the IAP antagonist Compound
A exhibits high binding affinity to the XIAP BIR3 domain. When Compound A was used at nontoxic concentrations in combination
with TRAIL, there was a significant increase in the sensitivity of TRAIL-sensitive and TRAIL-resistant bladder tumor lines
to TRAIL-mediated apoptosis. In addition, modulation of TRAIL sensitivity in the TRAIL-resistant bladder tumor cell line T24
with Compound A was reciprocated by … Source: MedWorm: Bladder Cancer

(Source: BJU International) Source: MedWorm: Bladder Cancer

(Source: BJU International) Source: MedWorm: Bladder Cancer

(Source: BJU International)

Source: MedWorm: Bladder Cancer

Bladder carcinoma is the second most common malignancy of the urinary tract and nearly 90% of all primary tumors of the bladder are urothelial carcinomas (UCs). Superficial UCs can be “shaved off” using an electrocautery device attached to a cystoscope. Immunotherapy in the form of bacillus Calmette-Guerrin (BCG) instillation is also used to treat and prevent the recurrence of superficial UCs, but there are still patients, whose UCs recurred after treatment with BCG. Therefore, the development of new drugs that target UC cells is desirable… (Source: Health News from Medical News Today) Source: MedWorm: Bladder Cancer

In this study, gas chromatography mass spectrometry (GC-MS) and two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOFMS) were employed for the metabolic footprinting of a pair of immortalized human uroepithelial cells namely HUC-1 (nontumorigenic) and HUC T-2 (tumorigenic). Both HUC-1 and HUC T-2 cell lines were cultivated in 1 mL of Ham’s F-12 media. Subsequent to 48 h of incubation, 200 muL of cell culture supernatant was protein-precipitated using 1.7 mL of methanol and an aliquot of 1.5 mL of the mixture was separated, dried, trimethylsilyl-derivatized, and analyzed using GC-MS and GCxGC-TOFMS. Metabolic profiles were analyzed using multivariate data analysis techniques to evaluate the changes of the metabolomes. Both GC-MS and GCxGC-TOFMS analyses showed disti… Source: MedWorm: Bladder Cancer

Homodimerization of RON (MST1R), a receptor tyrosine kinase, usually occurs in cells stimulated by a ligand and leads to the downstream activation of signaling pathways. Here we report that bladder cancer cells, in response to physiological stress, use an alternative mechanism for signaling activation. Time-course studies indicated that RON migrated directly from the membrane to the nucleus of bladder cancer cells in response to serum starvation. Biochemical and genetic studies implied that this nuclear internalization was complexed with epidermal growth factor receptor (EGFR) and required the docking of importins. In vivo analysis confirmed that nuclear RON was present in 38.4% (28/73) of primary bladder tumors. Chromatin immunoprecipitation (ChIP) on microarray analysis further revealed …

Source: MedWorm: Bladder Cancer

In conclusion, the double short-term exposure to bladder cancer cells by THP has more remarkable cytotoxic effects than the
single exposure in vitro.

Content Type Journal ArticleCategory Original ArticleDOI 10.1007/s10156-010-0088-yAuthors
Takuo Maruyama, Hyogo College of Medicine Department of Urology 1-1 Mukogawa-cho Nishinomiya Hyogo 663-8501 JapanYoshihide Higuchi, Hyogo College of Medicine Department of Urology 1-1 Mukogawa-cho Nishinomiya Hyogo 663-8501 JapanToru Suzuki, Hyogo College of Medicine Department of Urology 1-1 Mukogawa-cho Nishinomiya Hyogo 663-8501 JapanJun Qiu, Hyogo College of Medicine Department of Urology 1-1 Mukogawa-cho Nishinomiya Hyogo 663-8501 JapanShingo Yamamoto, Hyogo College of Medicine Department of Urology 1-1 Mukogawa-cho Nishinomiya Hyogo 663-850… Source: MedWorm: Bladder Cancer

Authors: Kim EY, Seo JM, Kim C, Lee JE, Lee KM, Kim JH
Aggressive bladder cancer is a major cause of morbidity and mortality. Despite the fact that metastatic disease results in death in the majority of bladder cancer cases, the molecular events regulating the invasive phenotype of aggressive bladder cancer are not well understood. In the present study, immunohistochemical examination showed that the leukotriene B(4) receptor BLT2 is overexpressed in advanced malignant bladder cancers [human transitional cell carcinomas (TCCs)] in proportion to advancing stages with high prognostic significance (p<0.001). Blockade of BLT2 with the specific antagonist LY255283 or siRNA knockdown significantly suppressed the invasiveness of highly aggressive 253J-BV bladder cancer cells. Moreover, our… Source: MedWorm: Bladder Cancer